Overview

With more than 4,000 recording sites sampled at 25 kHz, the CMOS chip allows extracellular recordings at very high resolution. The possibility to visualize signal propagation even on the sub-cellular level especially qualifies the CMOS-MEA5000-System for experiments interested in network functionality.

Cell Culture

Neuronal Networks

Dissociated cultures can be grown from primary or stem cell derived neurons and their activity studied on the CMOS-MEAs. Signals from hundreds or thousands of cells as well as signal propagation from cell to cell can be analyzed.

CMOS-MEA Application Note Neuronal Cultures
neuronal-culture
Optical image of a neuronal cell culture
Electrical image of a neuronal cell culture

Spike Sorting

The unique spike sorting implemented in the CMOS-MEA-Tools software takes advantage of the redundant information gathered by multiple sensors recording the signals of an individual cell. This allows fully automated, unsupervised spike sorting. The user can modify all parameters of the algorithm manually, or adjust the sensitivity with a simple slider interface.

Fully automated and easy to use spike sorting of a neuronal culture with one click

Retina

Axonal Signal Propagation

Using Spike Triggered Averages, it is possible to isolate repetitive events associated with spikes recorded on a specific channel. This method will enhance small signals often hidden in the noise and make, for example, signals traveling along axons and dendrites visible. This allows you to map functional connections along the sensor area.

Propagation of an average action potential along a single unmyelinated axon of a rat ganglion cell.
Details can be found in the paper of H.Stutzki et al. in Frontiers Cell. Neurosci. 8:38 (2014).

Light Induced Spiking Activity

Spiking activity upon presentation of different light stimuli (from top to bottom: deep blue (405 nm), blue (470 nm) and red light (640 nm). The light was flashed on at time 0.5 sec / 1.5 sec and 2.5 seconds.

CMOS-MEA Application Note Retina

Brain Slice

Activity Over Large Brain Areas

Not only spikes, but also traveling waves of LFPs, can be mapped so precisely that it would be possible to guess the anatomy of a slice preparation just from the recorded activity.

Bicuculline induced activity of an organotypic hippocampal slice